The deep transverse perenei are muscles of the pelvic diaphragm running transversely across the pelvic floor. They are continuous with the sphincter urethrae muscle anteriorly. They originate from the medial surface of the ischiopubic ramus. They insert into a midline raphe and the perineal body.
In females, the deep transverse perinei are traversed in the midline by the vagina.
They act to support the pelvic viscera and support the perineal body.
The deep transverse perinei are innervated by the perineal branch of the pudendal nerve (S2-S4).