Phosphoglycerate kinase is an enzyme of the glycolytic pathway which converts 3-phosphoglyceroyl phosphate into 3-phosphoglycerate.

For each molecule of 3-phosphoglyceroyl phosphate hydrolyzed, 1 molecule of ATP is produced from ADP; hence, for each glucose molecule substrate for glycolysis, 2 molecules of ATP are produced by this step.

The reverse reaction occurs in gluconeogenesis; it consumes 1 molecule of ATP per molecule of glucose formed.